2. Signaling Pathways
  3. Metabolic Enzyme/Protease
  4. Ser/Thr Protease

Ser/Thr Protease

Serine proteases; Serine endopeptidases; Threonine proteases

Ser/Thr Protease

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Serine (Ser) proteases catalyse the hydrolysis of specific peptide bonds in their substrates and this activity depends on a set of amino acids in the active site of the enzyme, one of which is always a serine. There are two families especially well studied, the trypsin family and the subtilisin family. Serine proteases play crucial roles in a wide variety of cellular as well as extracellular functions, including the process of blood clotting, protein digestion, cell signaling, inflammation, and protein processing. Threonine (Thr) proteases are a family of proteolytic enzymes harbouring a threonine residue within the active site. The prototype members of this class of enzymes are the catalytic subunits of the proteasome, however the acyltransferases convergently evolved the same active site geometry and mechanism.

Cat. No. Product Name Effect Purity Chemical Structure
  • HY-150070
    Butabindide
    		Inhibitor
    Butabindide (UCL-1397) is a potent, selective tripeptidvl peptidase II (TPP II) inhibitor with Ki values of 7 nM and 10 μM for TPP II and TPP I, respectively. Butabindide inhibits TPP II to protect CCK-8 against inactivation.
    Butabindide
  • HY-E70566
    FabCOUPER protease
    FabCOUPER protease is a serine protease expressed recombinantly in E.coli with a 6×His tag at the C-terminus. FabCOUPER protease specifically digests human IgG1 at a single site above the hinge, generating intact Fab and Fc fragments within two hours without reducing conditions.
    FabCOUPER protease
  • HY-169250
    TMPRSS13-IN-1
    		Inhibitor
    TMPRSS13-IN-1 (N-0430) is a potent TMPRSS13 inhibitor with a Ki of 25 nM.
    TMPRSS13-IN-1
  • HY-P34013
    Urinary Trypsin Inhibitor Fragment
    		Inhibitor
    Urinary Trypsin Inhibitor Fragment is a fragment derived from urinary trypsin inhibitor by proteolysis. Urinary Trypsin Inhibitor Fragment can inhibit tumor cell invasion by limited proteolysis.
    Urinary Trypsin Inhibitor Fragment
  • HY-W699983
    Imidazole-15N2
    		Inhibitor
    Imidazole-15N2 (Glyoxaline-15N2) is 15N labeled Imidazole. Imidazole (Glyoxaline; 1,3-Diaza-2,4-cyclopentadiene) is a heterocyclic aromatic compound. Imidazole bearing molecules have been used as corrosion, acetylcholinesterase (AChEI) and xanthine oxidase (XO) inhibitors, performing biological activities such as antifungal, antituberculosis, anti-inflammatory, antioxidant, and analgesic, amongst many others. Imidazole inhibits the enzymatic conversion of the endoperoxides (PGG2 and PGH2) to thromboxane A2 by platelet microsomes. Imidazole derivatives exhibits inhibition on SARS-CoV-2 3CLPro enzyme, which is promising for research in the field of Alzheimer’s disease, gout, COVID-19 and thrombo-embolic disease.
    Imidazole-<sup>15</sup>N<sub>2</sub>
  • HY-101959A
    Tyrphostin 47
    		Inhibitor
    Tyrphostin 47 is a nonspecific inhibitor of WNK1. Tyrphostin 47 is a protein tyrosine kinase inhibitor. Tyrphostin 47 inhibits smooth-muscle cell proliferation
    Tyrphostin 47
  • HY-118459
    L 658758
    		Inhibitor
    L 658758 is a potent inhibitor of serine proteinase.
    L 658758
  • HY-163184
    DCLK1-IN-3
    		Inhibitor
    DCLK1-IN-3 is a potent and selective doublecortin-like kinase 1 (DCLK1) inhibitor with an IC50 of 47 nM. DCLK1-IN-3 can be used for the research of cancer.
    DCLK1-IN-3
  • HY-153538
    AP-C2
    		Inhibitor
    AP-C2 is a potent guanosine 3',5'-cyclic monophosphate (cGMP)-dependent protein kinase II (cGKII) inhibitor with a pIC50 of 5.2.
    AP-C2
  • HY-174380
    DPP-1-IN-1
    		Inhibitor
    DPP-1-IN-1 (Compound 14) is an orally active inhibitor of Cathepsin C (also known as Dipeptidyl peptidase I) with an IC50 of 5.31 nM. DPP-1-IN-1 can inhibit the activity of Cathepsin C downstream Neutrophil elastase in neutrophils. DPP-1-IN-1 can be used in the research of inflammatory diseases.
    DPP-1-IN-1
  • HY-148415
    Ac-Phe-Gly-pNA
    Ac-Phe-Gly-pNA is the chymotrypsin specific substrate.
    Ac-Phe-Gly-pNA
  • HY-E70389
    Human Kallikrein
    Human Kallikrein is a serine protease that can be found in plasma and tissue. Human Kallikrein has the potential for the research of blood pressure, complement activation, and mediation and maintenance of inflammatory responses.
    Human Kallikrein
  • HY-129407A
    AAF-CMK
    		Inhibitor
    AAF-CMK is a TPPII (tripeptidylpeptidase II) inhibitor, shows anti-tumor activity and induces apoptosis. AAF-CMK can be used in leukemia research.
    AAF-CMK
  • HY-172090
    MASP-2-IN-1
    		Inhibitor
    MASP-2-IN-1 (Compound 77) is the inhibitor for mannan-binding lectin-associated serine protease (MASP), that inhibits MASP2 and MASP3 with IC50 of 11.4 nM and 13.2 µM. MASP-2-IN-1 exhibits a weak inhibitory activity against hERG with an IC50 of 175 µM. MASP-2-IN-1 reveals a poor stability in mouse plasma with a half-life of 4.4 hours.
    MASP-2-IN-1
  • HY-167924
    Ono 3307 free base
    Ono 3307 Free base is a novel synthetic protease inhibitor that exhibits protective effects against acute pancreatitis by preventing hyperamylasemia and pancreatic edema. Ono 3307 Free base also inhibits the redistribution of lysosomal enzymes in acinar cells and mitigates lactic dehydrogenase discharge. Ono 3307 Free base effectively reduces cathepsin B leakage from lysosomes in a dose-dependent manner. Ono 3307 Free base is able to target trypsin (Ki=48 nM), thrombin (Ki=0.18 μM), plasma kallikrein (Ki=0.29 μM), plasmin (Ki=0.31 μM), pancreatic kallikrein (Ki=3.6 μM), and chymotrypsin (Ki=47 μM).
    Ono 3307 free base
  • HY-P4528
    Ala-Phe-Pro-βNA
    Ala-Phe-Pro-βNA is the subsrate of prolyl tripeptidyl aminopeptidase, which is from Porphyromonas gingivalis. Ala-Phe-Pro-βNA binds to H-Ala-Ile-pyrrolidin-2-yl boronic acid for PTP39 and the E636A mutant with Ki values of 88.1 nM and 48.8 nM, respectivley.
    Ala-Phe-Pro-βNA
  • HY-E70586
    Elastase (high purity)
    Elastase (high purity) is a serine protease that hydrolyzes proteins and peptides.
    Elastase (high purity)
  • HY-P4366
    Tos-Gly-Pro-Lys-AMC
    Tos-Gly-Pro-Lys-AMC is a fluorogenic peptide substrate of tryptase.
    Tos-Gly-Pro-Lys-AMC
  • HY-145292
    TNIK-IN-2
    		Inhibitor 99.68%
    TNIK-IN-2 is a Traf2- and Nck-interacting protein kinase (TNIK) inhibitor, with an IC50 of 1.3337 μM. TNIK-IN-2 can be used for the study of colorectal cancer (CRC).
    TNIK-IN-2
  • HY-P4332
    Boc-Glu(OBzl)-Ala-Arg-AMC
    		Substrate
    Boc-Glu(OBzl)-Ala-Arg-AMC is a fluorogenic substrate for coagulation factor XIa and trypsin. The cleavage of the amide bond between arginine and the methylcoumarin amide group releases fluorescent 7-Amino-4-methylcoumarin (HY-D0027).
    Boc-Glu(OBzl)-Ala-Arg-AMC
Cat. No. Product Name / Synonyms Application Reactivity